Spectrophotometer manufacturer's spectrophotometer is a commonly used instrument in physical and chemical analysis.
Release time：2019-03-19
Spectrophotometer manufacturer's spectrophotometer composition:Spectrophotometer has become a conventional instrument in modern molecular biology laboratory. It is often used to quantify nucleic acid, protein and bacterial growth concentration. The instrument mainly consists of light source, monochromator, sample room, detector, signal processor and display and storage system.
Method and steps of using spectrophotometer:
1) Preheating instrument. In order to make the determination stable, the power switch is turned on and the instrument is preheated for 20 minutes. In order to prevent the fatigue of the photocell, no continuous illumination is required. When preheating the instrument and when not measuring, the cover of the dark box of the colorimetric dish should be opened to cut off the light path.
2) Selected wavelength. According to the requirements, the wavelength regulator is rotated so that the pointer indicates the required monochrome wavelength.
3) Fixed sensitivity file. In order to make the absorbance reading 0.2-0.7, the appropriate sensitivity is selected according to the absorption of light by the colored solution. For this reason, the sensitivity gear is rotated so that it can be fixed in a certain gear, and it will not change during the experiment. General measurements are fixed in "1" gear.
4) Adjust the "0" point. Turn the "0" potentiometer gently, so that the reading head pointer is located exactly at the "0" transmittance (at this time, the dark lid of the colorimetric dish is opened, the light path is cut off, and the phototube is not illuminated).
5) T = 100%. The colorimetric dish containing distilled water (or blank solution or pure solvent) is placed in the * compartment of the colorimetric dish rack. The colored solution is placed in other compartments. The lid of the dark box of the colorimetric dish is lightly covered. The light transmittance T = 100% is rotated to make the transmittance T = 100%, that is, the head pointer is exactly at T = 100%.
6) determination. Gently pull the tie rod of the colorimetric dish holder to let the colored solution enter the light path. At this time, the indicator on the watch head shows the absorbance A of the colored solution. After reading, open the lid of the dark box of the colorimetric dish.
7) shutdown. After the experiment, cut off the power supply, take out the colorimetric dishes and clean them with soft paper.
The spectrophotometer manufactured by the spectrophotometer manufacturer is a commonly used instrument in the physical and chemical analysis of zui. Its basic principle is based on the interaction between light and matter. When a photon collides with a substance molecule absorbing radiation in a solution, it absorbs. Measuring its absorbance value can reflect the quantity of a substance. The absorption of light is proportional to its concentration, which is the famous Bizhi law. The necessary condition for this law is that the sample is irradiated by monochromatic light (single wavelength light). In order to make the law have good linearity, there is a certain range requirement for measuring concentration.
Spectrophotometer manufacturer's spectrophotometer has become a conventional instrument in modern molecular biology laboratory. It is often used to quantify nucleic acid, protein and bacterial growth concentration. The instrument mainly consists of light source, monochromator, sample room, detector, signal processor and display and storage system.
